Forward chemical genetics compound libraries

Fig. 6-20 Forward chemical-genetic screen for inhibitors of protein deacetylation (data from Ref. 80). (a) Overview of cell-based screens of the 1,3-dioxane-based, diversity-oriented synthesis-derived library using antibodies to measure tubulin and histone acetylation, (b) Relative position of selected active compounds in a three-dimensional principal component model computed from five cell-based assay descriptors. AcTubulin-selective (red),...

In forward chemical genetics, having found a compound from the library, a hit , that elicits the desired phenotype, the final step is to identify its bio-molecular point of action. This is usually the biggest hurdle, especially if the target is novel. 

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