Forsythia intermedia

Fig. 12.6 Enantioselective formation of pinoresinol. Forsythia intermedia dirigent protein mediates selective formation of (-l-)-pinoresinol...

Fig. 12.7 Reactions catalyzed by pinoresinol/lariciresinol reductases from Forsythia intermedia (PLR-Fil) [34], Thujaplicata (PLR-Tpl and PLR-Tp2) [37], Liram album (PLR-Lal) [41], Linum perenne (PLR-Lpl) [42], and Linum usitatissimum (PLR-Lul) [41] and pinoresinol reductases from Arabidopsis thaliana (AtPrRl and AtPrR2) [55]...

Katayama T, Davin LB, Lewis NG (1992) An extraordinary accumulation of (-)-pinoresinol in cell-free extracts of Forsythia intermedia evidence for enantiospecific reduction of (+)-pinoresinol. Phytochemistry 31 3875-3881... 

Dinkova-Kostova AT, Gang DR, Davin LB et al (1996) (-l-)-Pinoresinol/(-l-)-lariciresinol reductase from Forsythia intermedia. J Biol Chem 271 29473-29482... 

Umezawa T, Davin LB, Lewis NG (1991) Formation of lignans (-)-secoisolariciresinol and (-)-matairesinol with Forsythia intermedia ceU-free extracts. J Biol Chem 266 10210-10217 Okunishi T, Sakakibara N, Suzuki S et al (2004) Stereochemistry of matairesinol formation by Daphne secoisolariciresinol dehydrogenase. J Wood Sci 50 77-81 XiaZ-Q, Costa MA, Pelissier HC et al (2001) Secoisolariciresinol dehydrogenase purification, cloning, and functional expression. J Biol Chem 276 12614-12623... 

Umezawa T, Davin LB, Lewis NG (1991) Formation of the lignans (-) secoisolanciresinol and (-)-matairesinol with Forsythia intermedia cell-free extracts J Biol Chem 266(16) 10210-10217... 

Dinkova-Kostova, A.T., Gang, D.R., Davin, L.B., Bedgar, D.L., Chu, A. and Lewis, N.G. (1996) (+)—Pinoresinol/(+)—lariciresinol reductase from Forsythia intermedia protein purification, cDNA cloning, heterologous expression and comparison to isoflavone reductase.. Biol. Chem., 46,29473-82. 

Fig. 8.8 Expression of lignan In transgenic rice endosperm. (A) LIgnan biosynthesis pathway derived from Forsythia intermedia [35]. (B) Matalresinol level In transgenic gram (4PE-256) and non-transgenic wild-type (TP309). GC/MS scan shows accumulation of lignan In transgenic rice endosperm. (C) Matalresinol...

Amanoa oblongifolia Forsythia intermedia Ipomea cairica Pimts sp. 

Figure 3. Biosynthetic pathway from -coniferyl alcohol 7 to (-)-matairesinol 24b in Forsythia intermedia...

The plant systems adopted as biological partners were the Amazonian species, Virola surinamensis and the temperate plant, Forsythia intermedia, respectively. The latter accumulates the 8,8 -linked, optically pure lignans (-)-matairesinol 36b and (-)-arctigenin 74, whereas V. surinamensis affords surinamensin 50. Preliminary studies using either plants or cell-free extracts of V. surinamensis from ca one- to two-year-old plants and eugenol 40/ isoeugenol 41/[8-14C]coniferyl alcohol 13 as precursors did not result in formation of either surinamensin 50 or virolin 51 under the conditions employed. 

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