Forrest reagent

Fig 1 Chromatogram of imipramine and phenothiazine derivatives after staining with Forrest reagent [4] 1 = imipramine, 2 = desipramine, 3 = clomipramine, 4 = lofepramine, 5 = trimipramine, 6 = thioridazine, 7 = chlorphenethazine, 8 = periciazine, 9 = promazine, 10 = promethazine. 

Table 11 Colors of chromatogram zones after treatment with Forrest reagent [1].

Note The colors obtained are characteristic for the various substance classes (Table 1). Thus color tones obtained for phenothiazine derivatives are mainly reddish [1], If the j chromatogram zone contains more than 10 pg substance per spot there is only a colored 1 outer ring surrounding an uncolored center (Fig. 1) [1, 4]. The Forrest reagent does not interfere with subsequent detection with the Dragendorff reagent [1, 4], 1... 

Carry out die test as described for urine (above) using a suitable volume of the filtered sample. 6.1mipramine, desipramine, and trimipramine— Forrest reagent. Carry out the test as described for urine (above) using a suitable volume of the filtered sample. 

Forrest Reagent FPN Reagent Liebermann s Test Mandelin s Test Marquis Test Nessler s Reagent Sulphuric Acid... 

Indications. The indications for this reagent are the same as those stated for Forrest Reagent. 

Colour Tests. Formaldehyde-Sulphuric Acid—blue-violet Forrest Reagent—red FPN Reagent—brown-orange Lieber-mann s Test—brown Mandelin s Test—green—> red Marquis Test—yellow-green— red. 

Colour Tests. Formaldehyde-Sulphuric Acid—blue Forrest Reagent—red FPN Reagent—red-orange Liebermann s Test—red-brown Mandelin s Test—red-violet Marquis Test— orange - red-violet. 

Colour Tests. Formaldehyde-Sulphuric Acid—red-violet Forrest Reagent—red FPN Reagent—red Liebermann s Test— red-brown Mandolin s Test—green-> violet Marquis Test— violet. 

Colour Tests. Forrest Reagent—blue FPN Reagent—blue Liebermann s Test—blue Mandelin sTest—blue. 

Colour Tests. Formaldehyde-Sulphuric Acid—violet Forrest Reagent—red FPN Reagent—red Mandelin s Test—green-brown Marquis Test—red- violet Sulphuric Acid—violet- blue. 

Colour Tests. Formaldehyde-Sulphuric Acid-red-violet Forrest Reagent—red-orange FPN Reagent—red Liebermann s Test—green-brown Mandelin s Test—greenviolet Marquis Test—red-violet. 

Colour Tests. Formaldehyde-Sulphuric Acid—red Forrest Reagent—brown FPN Reagent—orange Mandelin s Test— green Marquis Test—red Sulphuric Acid—red. 

Colour Tests. Ferric Chloride—green Formaldehyde-Sulphuric Acid—red-violet Forrest Reagent—orange FPN Reagent— orange Liebermann s Test—green Sulphuric Acid—green. 

Colour Tests. Forrest Reagent—violet- red- orange FPN Reagent—violet-red - red - orange Mandelin s T est—or-... 

Colour Tests. Formaldehyde-Sulphuric Acid—green Forrest Reagent—blue FPN Reagent—blue Mandelin sTest—violet Marquis Test—red green. 

Colour Tests. Formaldehyde-Sulphuric Acid—pink Forrest Reagent—orange FPN Reagent—orange Liebermann s Test—red Mandelin s Test—red-brown Marquis Test—red-violet. 

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