Formylglycinamide ribonucleotide

OXALYL-CoA DECARBOXYLASE N-Formylglutamate transformylase, GLUTAMATE FORMYLTRANSFERASE FORMYLGLYCINAMIDE RIBONUCLEOTIDE AMIDOTRANSFERASE FORMYLGLYCINAMIDINE SYNTHETASE... 

These reactions suggest the corresponding biochemical ones see Equation 13). In the presence of pyruvate kinase, ATP and pyruvate are equilibrated with phosphoenolpyruvate and ADP 74, 75, 76). Furthermore, in the presence of formylglycinamide ribonucleotide amidotransferase, ATP reacts with 5-phosphoribosyl-N-formylglycin-amide and glutamine to yield 5-phosphoribosyl-N-formylglycinami-dine according to Equation 14 (77). 

Formate is activated and then added to this amino group to form formylglycinamide ribonucleotide. In some organisms, two distinct enzymes can catalyze this step. One enzyme transfers the formyl group from N lO-formyltetrahydrofolate (Section 24.2.6). The other enzyme activates formate as formyl phosphate, which is added directly to the glycine amino group. 

AIR. /-(S-O-Phosphono-d-D-ribofuranosyl)-lH-imidazol-5-amine 5-amino-I-ribofuranosylimidazole S -phosphate 5-aminoimidazole ribonucleotide 5-aminoimida-zole ribotide 5 -amino -1 -(5 -phosphofuranoribosy] )imida -zole. C H14N307P mol wt 295-19. C 32.55%. H 4.78%, N 14.23%, O 37.94%, P 10.49%. An intermediate in the biosynthesis of purines. Synthesis from formylglycinamide ribonucleotide Levenberg, Buchanan, J. Biol Chem. 224, 1005 (1957) Lukens, Buchanan, ibid. 234, 1791 (1959). 

The conversion of formylglycinamide ribonucleotide (FGAR) into formylglydnamidine (FGAM) is the fourth step in the de novo biosynthesis of purines. This reaction is catalyzed by formylglycinamide ribonucleotide... 

The rate of purine synthesis in these patients fibroblasts, as estimated by the accumulation of l C-formate into formylglycinamide ribonucleotide (PGAR) in the presence of azaserine, is increased... 

Figure 2 Physiology and metabolism of folate. GAR, glycinamide ribonucleotide FGAR, formylglycinamide ribonucleotide AlCAR aminoimidazolecarboxamide ribonucleotide figlu, formiminoglutamic acid IMP, inosine monophosphate.

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