Formation of the Lead—Group-IIA Bond

It has been shown that inactivation of pristinamycin IIa by a plasmid-mediated resistant strain of Staphylococcus aureus, which was able to produce an acetyl transferase, occurred as a result of enzymatic acetylation of the allylic hydroxy group [19, 20]. The same resistant strain of Staphylococcus aureus was also demonstrated [21] to be capable of inactivating pristinamycin Ia by enzymatic hydrolysis of the ester bond in the macrocycle leading to formation of the linear hexapeptide, which although still capable of tight binding to the ribosome was antibacterially inactive. 

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