Format or Single-Channel Method

We use two subscripts to indicate the orientation of the citation and emission polarizers, respectively. For example, Iffy corresponds to horizontally polarized excitadon and vertically polarized emission. This notation is easy to recall since the order of the subscripts represents the order in which the light passes through the two polarizers. 

Note that it does not matter if the intensity of the excitation is changed when the excitation polarizer is rotated to the horizontal position. This change is a constant factor in the numerator and denominator of Eq. [10.31], and hence cancels. When the G-factor is known, the ratio Mix can be calculated using 

To calculate the actual intensity ratio (Mix), we need to determine the G-factor, which is the ratio of the sensitivities of the detection syston for vertically and horizontally polarized light. 

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