Fluorimetric reagent

Avigad, G., Dansyl hydrazine as a fluorimetric reagent for thin-layer chromatographic analysis of... 

Two features of luminescence make it a powerful method for analytical detection of metal ions. First is sensitivity fluorimetric reagents have been developed for the detection of even nanomolar metal ion concentrations. Indeed, even single molecule fluorescence is possible. 

Second is selectivity fluorimetric reagents may bind selectively to only particular metal ions, or only particular metal ions may lead to changes in luminescence of the ligand. 

Procedure 7.1.b Designing Experiments to Investigate the Use of HQS as a Fluorimetric Reagent for Metal Ion Detection... 

The objective of this exercise is to design meaningful experiments to investigate the potential use of HQS as a fluorimetric reagent for trace metal ions. Changes in luminescence properties that you can measure include quantum efficiency, wavelength maximum (Amax), and/or band shape of the emission. Keep in mind that many factors can affect the luminescence intensity of a sample. In a well-designed experiment, the effect that a particular variable (metal ion, temperature, pH, etc.) has on the HQS fluorescence intensity needs to be separated from other effects. 

Or do only some metal ions affect fluorescence (a trait of a specific fluorimetric reagent) ... 

Could HQS serve as a fluorimetric reagent for the quantitative analysis of a particular metal ion ... 

In Table 3 are compiled data on recent fluorimetric determinations of elements124, 126,128,140,143 289) and in Table 4 the fluorimetric reagents most widely used in inorganic analysis. 

Table 4. Most widely used fluorimetric reagents in inorganic analysis...

The use of fluorimetric reagents generally increases the sensitivity of detection by 1 to 2 orders of magnitude. Hirayama (1974) used anthrone for fluorimetric determination of pentoses and 5-hydroxy-l-tetralone for hex-oses. However, sodium chloride was found to interfere, thus necessitating an additional desalting step. Concentrated sulphuric acid was used in both determinations and thus the method has its drawbacks and interferences as stated above. Furthermore, an increase in the natural fluorescence background of seawater samples may occur on heating and lead to overestimations. 

Spectrophotometric procedures, including absorptimetric and fluo-rimetric, will continue to be popular and important because they can be sensitive and accurate, and because the necessary instruments are now famihar laboratory tools. Intrinsically, fluorimetric methods can be several orders of magnitude more sensitive than absorptimetric. To date, most direct fluorimetric methods are for the determination of metals, while anions are determined by their quenching action on fluorescent compounds. Hopefully, other new direct fluorimetric reagents for anions similar to the one described above for cyanide ion using quinone monoxime benzene sulfonate ester will be developed either as the result of chance observations or the application of increased fundamental knowledge of the fluorescence process. There probably will be few if any major advances in the quality of the instruments used in these methods improved methods mainly will be the result of more sensitive and more selective analytical reagents. 

Sabbioni, C. Raggi, M. A. Casamenti, G. Varani, G. Zini, Q. Comparison of fluorimetric reagents for the analysis of aluminum in mineral waters. Anal. Lett. 1999, 32, 123-137. 

Fluorimetric reagent for detn. of carbonyl compds. and reducing sugars. Mp 128-130°. 

Diehl, H., Titrimetric, Colorimetric and Fluorimetric Reagents for Ca and Mg, G. Frederick Smith Co., Columbus, Ohio, 1964 use)... 

Fluorimetric reagent for aromatic aldehydes and a-ketoacids. Prisms. Mp 131-132°. 

Fluorimetric reagent for hexoses used in detn. of blood sugar. Light yellow cryst. Mp 209-211°. 

Fluorimetric reagent for amino acids, primary amines, peptides, proteins and proteolytic enzymes. Cryst. (CH2Cl2/Et20). Mp 154-155°. 

Fluorimetric reagent for a-keto acids and neuraminic acids. Pale yellow prisms (propanol). Sol. EtOH,... 

Fluorimetric reagent for amines. Bright yellow-orange needles (EtOH). Mp 151°. 

Fluorescent indicator for intracellular Ca. Tetrakis acetoxymethy[)ester [83104-85-2]. Quin 2A C38H43N3O1 M 829.767 Fluorimetric reagent for Ca. 

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