Fluorescent timer

The fluorescent timer is another DsRED mutant with the unique ability to change its light emission peaks from green to red over time. This property results from the pointmutations VI05A and S197T present in fluorescent timer [32],... 

Mature wildtype DsRED protein and its recombinant derivatives DsRED2, DsRED.Tl, DsRED.T3 DsRED.T4 proved to be stable (soluble and fluorescent) at temperatures up to 70-75 °C, Fig. (19). For RedStar, mRFPl, Fluorescent timer , mcavRFP, HcRED and equaRFPl (eqFP611) such data currently are not available. 

Alexey Terskikh, Arkady Fradkov, Galina Ermakova, Andrey Zaraisky, Patrick Tan, Andi ey V. Kajava, Xiaoning Zhao, Sergey Lukyanov, Mikhail Matz, Stuart Kim, Irving Weissman and Paul Siebert, Fluorescent Timer protein that changes color with time. Science, 290 (2000), 1585-1588. 

Where K is an instrumental constant- In this expression t corresponds to the macroscopic evolution of the sample whereas u corresponds to a microscopic reorientational motion in the scale of the fluorescence lifetime T - In most cases the to dependence of N and P can be ignored within the timer (ca.10 s), and the fluorescence intensity emitted under continuous excitation is given by ... 

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