Fluorescent sensors main classes

CEF Chelation or Complexation Enhancement of Fluorescence CEQ Chelation or Complexation Enhancement of Quenching Fig. 10.1. Main classes of fluorescent molecular sensors of ions or molecules. 

From a general point of view, a chemical sensor is a device capable of continuously monitoring the concentration of an analyte. The two main classes are electrochemical sensors and optical chemical sensors. The latter are based on the measurement of changes in an optical quantity refractive index, light scattering, reflectance, absorbance, fluorescence, chemiluminescence, etc. For remote sensing, an optical fiber is used, and the optical sensor is then called an optode because of... 

Figure 16.1 Main classes of fluorescent molecular sensors of ions and molecules based on (a) quenching of luminescence by collisions with analyte (b) quenching or (c) increase of fluorescence of the complexing fluorophore (d,e) the same processes in fluorophore-receptor assemblies [1]...

Fluorescent probes have been extremely useful in elucidating biochemical mechanisms and processes inside of living cells via fluorescent microscopy. This technique is particularly valuable because it is non-destructive and the probes can be observed in real time over the course of cellular events. Fluorescent probes fall into two main classes chemosensors and biosensors. Biosensors are fluorescently labelled proteins, most often antibodies. These types of probes have the disadvantage of poor cell permeability, but can be generated with specificity for any macromolecule against which an antibody can be raised. Chemical sensors are typically based on synthetic compounds and have been used in cells mainly to quantify the concentration of certain... 

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