Fluorescent ligand , molecular assembly

Fig. 1. Various schematics of bead display for molecular assemblies on beads. The Py subunits of the G protein (circles labeled with [i and y) are fused with either FLAG or hexahistidine tag, which recognizes the biotinylated M2 anti-FLAG antibodies on streptavidin-coated beads or chelated nickel on the dextran-treated beads. A socket and plug connecter is utilized to depict the very high-affinity interaction of the epitope tag. This modular setup allows for either a subunit (for capturing FPR-GFP) or as subunit (for capturing / 2AR-GFP) to be coupled with the fly subunit to form the complete G protein coating the bead. Fluorescent components such as GFP or ligand are indicated in green. See text for details.

Sklar, L. A., Vilven, J., Lynam, E., Neldon, D., Bennett, T. A., and Prossnitz, E. (2000). Solubilization and display of G protein-coupled receptors on beads for real-time fluorescence and flow cytometric analysis. Biotechniques 28, 976-980, 982-985. Sklar, L. A., Edwards, B. S., Graves, S. W., Nolan, J. P., and Prossnitz, E. R. (2002). Flow cytometric analysis of ligand-receptor interactions and molecular assemblies. Annu. Rev. Biophys. Biomol. Struct. 31, 97—119. 

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