Fluorescent carbohydrate conjugates

During this period, Laurie also investigated the preparation of radiolabeled, iodo and bromo sugars via carbohydrate boranes (with J.-R. Neeser), fluorescent probe-sugar conjugates (with M. Yalpani), sugar ferrocene derivatives (with M. J. Adam), and extended his studies of the conformations of polycyclic sugar derivatives. 

Probably the application of system peaks of most practical importance is the detection and quantitation of analytes which cannot be detected directly. Then, an additive that is easy to detect and whose concentration can readily be monitored is added to the mobile phase. In most cases, this method is applied for the detection of compounds that have no UV chromophores in the range of conventional UV absorption photometry (e.g., triglycerides and other lipids, carbohydrates), and a UV detector is used with a conjugated aromatic compoimd as additive. The method has been used also with fluorescence [24] and electrochemical detection [25]. 

Fig. 11.2-2 Tools for glycobiology a - modified surfaces for microarrays and surface plasmon resonance (SPR), b - monovalent fluorescent conjugates, c - neoglycoproteins and carbohydrate...

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