Flux, fluorescent-based

Limitations of Fluorescent-Based Flux or Membrane Potential Assays. .. 99... 

Fluorescent-based flux or membrane potential assays provide superior throughput compared to traditional electrophysiology-based screening technologies. For fluorescent-based membrane potential assays, however, the response to increasing channel activity can be non-linear due to the inherent non-linear relationship between channel activity and membrane potential (Fig. 4A). This prevents an accurate readout of efficacy and reduces the reso-... 

Fluorescence-based methods do not directly measure ionic current but, rather, measure either membrane-potential-dependent or ion-concentration-dependent changes of fluorescence signals (from fluorescent dyes loaded into the cytosol or cell membrane) as a result of ionic flux. Because fluorescence-based methods give robust and homogeneous cell population measurement, these assays are relatively easy to set up and achieve high throughput. 

Key words Cardiotoxicity, In vitro assays for cardiotoxicity, Cardiac safety, Functional flux assays, Fluorescence-based assays, hERG, Patch clamp, Alternative tests, Microelectrode array, hESC-derived cardiomyocytes, FLIPER, Q-T interval, HEK 293, CHO, Embryonic stem cells, Differentiation, hiPSCs, Drug interaction, Safety pharmacology, ICH guidelines, Arrhythmia... 

Li QY, Rottlander M, Xu MK et al (2011) Identification of novel KCNQ4 openers by a high-throughput fluorescence-based thallium flux assay. Anal Biochem 418(l) 66-72... 

Recent research has shown that phosphor-based TSPs could be used for high temperature thermometry applications. Fluorescence from YAGiEu and YAG Ce could be detected through several thin YSZ samples. The average prompt fluorescence decay time for YAGiCe was measured to be 62.7 zb 2.9 ns, which is close to the accepted value of 65 ns. This result can be used directly to develop an operational high temperature heat flux gauge. 

Fig. 7 (A) Phosphor-based heat flux gauge on turbine blade and (B) fluorescing gauge with matching triangular phosphor layers.

---