Fluorescence immunoassay with clonotype antibodies

Fluorescence and Enzyme Immunoassays with Clonotype Antibody. 92... 

M5. Miyai, K., Endo, Y., and Hata, N., Fluorescence immunoassay of thyroxine with clonotype antibody fractionated by ehromatofoeusing. Proc. Int. Fed. Clin. Chem., 12th, in press (1985). 

EDgQ for the initial antithyroxine was 9.2 x 10 9 M and the values for the fractions were in the range (6.3-13.0) x 10 9 M. However, the clonotype antibodies with which the most sensitive assay could be achieved differed for different types of immunoassays i.e., fractions I and VIII for radioimmunoassay, fraction I for fluorescence immunoassay, and fractions II, IV, and VIII for enzyme immunoassay in these experiments. The sensitivities of immunoassays may be improved by using appropriate clonotype antibodies. 

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