Fluorescein leakage

Clothier R, Starzec G, Pradel L et al. (2002) The prediction of human skin responses by using the combined in vitro fluorescein leakage/Alamar Blue (Resazurin) assay. ATLA. 30 493-504. 

Phase I and n trials of verteporfin demonstrated that CNVMs were occluded for 1 to 4 weeks after a single PDT session, with fluorescein leakage noted 4 to 12 weeks later. Because CNVM reperfusion occurred after a single PDT session, researchers evaluated the safety and efficacy of repeated treatments on CNVM stability. They discovered that retreatment at 2 or 4 weeks after the initial PDT still allowed for leakage to recur in most subjects at 4 to 12 weeks but to a lesser extent after multiple treatments. 

Fluorescein leakage (FL) Validated and adopted (OECD TG 460) Applicable to water-soluble substances and mixtures. 

Table 3 Grading of Extent of Fluorescein Leakage Following PDT...

The extent of post-treatment fluorescein leakage from CNV relative to baseline appeared to be correlated with visual acuity outcome. The 33 patients with lesions that showed a reduced area of leakage from both classic and occult CNV at 12 weeks, compared with baseline, had a mean change in visual acuity of +0.8 line. In comparison, the 49 patients who showed progression of either classic or occult CNV by 12 weeks lost 0.8 line of vision. This difference was statistically significant (P 0.012), suggesting that patients in whom stabilization of CNV leakage is achieved with PDT may have a beneficial visual outcome. 

Angiographic evaluation revealed absence of fluorescein leakage from classic CNV in 43% of patients at the month 12 visit. Progression of classic CNV was noted... 

Fluorescein leakage (FL) Monolayers or multi-layers of epidermal keratinoeytes Permeabihty Prevahdated... 

Flange, N. Bienert, M. Remky, A. Arend, K. O. Optic disc fluorescein leakage and intraocular... 

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