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Flp recombination target

Tyrosine recombinases of the lambda family also function in eukaryotes. Best known is the FLP (Flip) recombinase, which is encoded by the 2-pm plasmid of Saccharomyces cerevisiae and is thought to function in amplifying the number of plasmid copies.265 The 6.3-kbp plasmid contains a unique DNA sequence that lies between two 599-bp repeats in inverted orientation. Embedded in each repeat is an FLP recombination target (FRT) sequence, which is recognized by the plasmid recombinase. Each FRT segment includes inverted repeats 13 bp in length with an 8-bp spacer between them. As with other integrase systems the... [Pg.1572]

Analogously to the Cre-LoxP system, the 34 bp Flippase recognition target (FRT) sites are recognized by the Flippase recombination enzyme (FLP) derived from Saccharomyccs cerevisiae (28). The FLP-FRT recombination system is also utilized for the inducible genetic manipulation of the mouse genome (29, 30). [Pg.287]

See other pages where Flp recombination target is mentioned: [Pg.1572]    [Pg.57]    [Pg.536]    [Pg.659]    [Pg.659]    [Pg.638]    [Pg.18]    [Pg.141]    [Pg.403]    [Pg.1572]    [Pg.57]    [Pg.536]    [Pg.659]    [Pg.659]    [Pg.638]    [Pg.18]    [Pg.141]    [Pg.403]    [Pg.65]    [Pg.66]    [Pg.250]    [Pg.122]    [Pg.459]    [Pg.154]    [Pg.61]    [Pg.62]    [Pg.767]    [Pg.490]    [Pg.653]    [Pg.59]    [Pg.118]   
See also in sourсe #XX -- [ Pg.122 ]



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