Flow cytometry injury

COUPLING FLOW CYTOMETRY WITH IMAGE ANALYSIS-ASSESSING THE ROLE OF MACROPHAGES IN TISSUE INJURY... 

In sum, EMPs have emerged as a preferred direct method for assessing EC injury in different disorders. EMP analysis could provide insight into the actual status of the endothelium in vivo by a simple blood analysis. However, there is a need for refinement and standardization of the assay method. Overall, most groups have relied on flow cytometry for the measurement of EMPs nevertheless, other methods such as ELISA are available and may be an option in the future. The main challenge remains in the selection of specific and sensitive monoclonal antibodies that may yield consistent results between different laboratories. In addition, the protocols for sample handling and storage need to be clearly delineated. The assay is still a... 

Lovastin induced apoptosis in acute lung injury and idiopathic pulmonary fibrosis fibroblasts as determined by TUNEL, acridine orange staining, and flow cytometry (Tan et al. 1997). 

Luo, J. Li, N. Paul Robinson, J. Shi, R. Detection of reactive oxygen species by flow cytometry after spinal cord injury. J. Neurosci. Methods 2002, 120, 105-112. 

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