Flow cytometry immunofluorescence staining

Ladies, G.S., et al., Phase two of an interlaboratory evaluation of the quantification of rat splenic lymphocyte subtypes using immunofluorescent staining and flow cytometry, Toxicol. Methods, 8, 87, 1998. 

Successful cell surface display of the protein can be verified by inducing gene expression via addition of anhydrotetracycline to the culture medium and immunofluorescence staining of the cells using an antibody directed against the protein to be displayed (Protocol 1). Analysis can be performed by fluorescence microscopy or flow cytometry. 

Nusse M, Recknagel S and Beisker W (1992). Micronuclei induced by 2- chlorobenzylidene mal-ononitrile contain single chromosomes as demonstrated by the combined use of flow cytometry and immunofluorescent staining with anti-kinetochore antibodies. Mutagenesis, 7, 57-67. 

Visser, J. et al. Quantitative immunofluorescence in flow cytometry, in Immunofluorescence and Related Staining Techniques (eds.) Knapp, W. et al., p. 147, Amsterdam, Elsevier/ North-Holland, 1978... 

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