Flow-coating method

As stated in section 2.2.1 the joining of the microstructures to a form an assembled microreactor system can be before or after the coating, suggested denotation post-coating or pre-coating respectively. Due to non-accessible walls of the microstructure in the inner part of assembled microreactors only a flow coating method can be applied, either in the liquid phase (see Fig. 7) or from the gas phase. Procedures which can apply to the flow coating are... 

Badal, M.Y., Wong, M., Chiem, N., Salimi-Moosavi, H., Harrison, D.J., Developing a routine coating method for multichannel flow networks on a chip using pyrolyzed poly(dimethylsiloxane). Micro Total Analysis Systems, Proceedings 5th pZAS Symposium, Monterey, CA, Oct. 21-25, 2001, 535-536. 

Owing to its applications in homeland security, sterilization validation, and astrobiology, bacterial spore detection has become a hot field. However, direct detection of bacterial spores can be challenging for the same reasons that make endospores difficult to irradicate. The tough spore coat is impermeable to staining techniques, so most microscopy and flow cytometry methods are not useful. Endospores are also highly resistant to lysis, meaning that common DNA extraction protocols are difficult to perform. The lack of measurable metabolism renders microcalorimetry and cellular respiration techniques ineffective. 

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