Flow chamber sheath fluid

Fig. 1 Multiple-capillary instrument employing the sheath-flow technique. Key 14, capillary 18, capillary outlet 20, capillary inlet 22, buffer well 24, microtiter plate 26, quartz chamber 36, laser 38, laser beam 40, lens 58, fluidic stream. The electrodes are not shown nor is the device for delivering the sheath fluid. (Reprinted in part from U.S. Patent No. 5,741,412, Figure 1.)...

How cytometry involves particles or cells flowing through a laser beam. Signals are produced aud provide information about the particle. The process begins with an isotonic Uquid called sheath fluid, which euters the flow chamber from the side and flows downward in the Coulter EPICS Elite series flow cytometers... 

Fig. 3 The sheath-flow cuvette fluorescence detection chamber for an array of five capillaries. The chamber is tapered. A single laser beam is used to illuminate fluorescence from the five sample streams isolated by the sheath flow fluid.

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