Flavonoids cytochrome P450 monooxygenase

Cytochrome P450 monooxygenases play two important roles in flavonoid biosynthesis. One involves the modification of ring C of flavanones, where they attack nonphenolic carbon atoms, thus resulting in the creation of new flavonoid classes such as flavones, flavonols, and isoflavonoids. The other involves the introduction of new hydroxyl groups on phenolic rings A and B, thus increasing the... 

LATUNDI-DADA, A.O., CABELLO-HURTADO, F CZITTRICH, N., DIDIERJEAN, L., SCHOPFER, C., HERTKORN, N., WERCK-REICHHART, D., EBEL, J., Flavonoid 6-hydroxylase from soybean Glycine max L.), a novel plant cytochrome P450 monooxygenase. J. Biol. Chem., 2001,276,1688-1695. 

Fig. 5 Schematic representation of enzymatic reactions responsible for the modification of plant-derived secondary metabolite scaffolds, using phenylpropanoids/flavonoids as an example. Glc, glucose lesidue, 2-ODD, 2-oxoglutarate-dependent dioxygenase O-GT, O-glycosyltransferase OMT, O-methyltransferase, P450, cytochrome P450 monooxygenase ST, sulfotransfeiase...

Sortinl decomposes in plant growth media where one of the products resembles the effects of BSO, a GSH biosynthesis inhibitor. This indicates that a series of observed Sortinl effects might be due to an oxidized cellular environment that it creates. It could be that Sortinl or an active decomposition product sequesters GSH or inhibits GSH production. This may induce the transcription of cytochrome P450-dependent monooxygenases and mixed function oxidases, as shown in the transcription profile after Sortinl treatment. Reduced GSH level then affects flavonoid transport. 

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