Flavin mononucleotide adsorption

Flavin mononucleotide (Na, 2H2O salt, FMN) [130-40-5] M 514.4. Purified by paper chromatography using leri-butanol-water, cutting out the main spot and eluting with water. Also purified by adsorption onto an apo-flavodoxin column, followed by elution and freeze drying [Mayhew and Strating Eur J Biochem 59 539 1976]. 

Flavin nucleotides—flavin adenine dinucleotide (FAD) and flavin mononucleotide (riboflavin-5 -phosphoric acid) (FMN)—as prosthetic groups can either comprise a metal or serve as cofactors without a metal. Electrochemical transformations of FAD and FMN are characterized by strong adsorption. The area occupied by a FAD molecule on a mercury electrode is 280 A, i.e., close to the geometrical dimensions corresponding to the molecular model. Reduction of FAD and FMN proceeds in two reversible... 

C,7H2oN406 376.368 Widely distributed, but occurs naturally in free form only in the retina, in whey and in urine. Main forms occurring in tissues and cells are flavine mononucleotide and flavine-adenine dinucleotide. Colour additive in food. Used as adsorption indicator in titrimetric detn. of Ag. Nutrient supplement. Vitamin cofactor. Orange needles. Sol. EtOH spar. sol. H2O. Mp 280° dec. [ ]d -9.8 (H2O). [ ]d -125 (20A NaOH). pAa, 1.9 pA,2 10.2 (25°). Green fluor. in soln. X ax 220 265 365 455 (H2O). 

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