First-Generation CIEF-RPLC-MS System for Proteins

3 FIRST-GENERATION CIEF-RPLC-MS SYSTEM FOR PROTEINS 

In order to collect and analyze protein fractions sequentially, hydrodynamic flow must be cycled on and off. In the go mode, pressure is applied to the anodic end to push focused bands into the injection loop. In the stop mode, the pressure is removed so that the analytes remaining in the capillary do not move. After the collected fraction has been transferred to the trapping column and washed, the microselection valve switches back to its original configuration and pressure is reapplied to the anodic end to collect a new fraction. While the new fraction is being collected, the fraction loaded on the trap column is eluted and analyzed by RPLC-MS. 

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