First generation bio-adipic acid

14 g/L. Another new artificial biosynthetic route has been described for E. coli that uses besides glucose also glycerol as substrate. Anthranilic acid, the first intermediate in the blocked tryptophan biosynthetic branch, was subsequently converted to catechol and di,c -muconic acid (Sun et al., 2013). Genes for anthranilate 1,2-dioxygenase and catechol 1,2-dioxygenase were coexpressed fromP. aeruginosa and 

FIRST AND SECOND GENERATION PRODUCTION OF BIO-ADIPIC ACID 

Erytrose 4-phosphate acid Phosphoenoi pyruvic acid 

Process Strain Substrate Maximum specific productivity (g/g-dcwh) Maximum volumetric productivity (g/L-h) Final concentration (g/L) Molar yield % 

Shake flask cultures E. co/iBW25113 AtrpD kan Glucose and Glycerol nd 3 X 10-3 0.39 2.2 

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