Fingerprinting methodology

The ability to resolve and characterize complicated protein mixtures by the combination of 2DLC and online mass spectrometry permits the combination of sample fractionation/simplification, top-down protein mass information, and bottom-up peptide level studies. In our lab, the simplified fractions generated by 2D(IEX-RP)LC are digested and analyzed using common peptide-level analysis approaches, including peptide mass fingerprinting (Henzel et al., 1993 Mann et al., 1993), matrix-assisted laser desorption/ionization (MALDI) QTOF MS/MS (Millea et al., 2006), and various capillary LC/MS/MS methodologies (e.g., Ducret et al., 1998). 

Methodology In Figure 1 a) and b) the principles of catalytic profiling analysis are explained Catalytic profiling analysis includes a set of test reactions which are very sensitive with respect to catalyst properties and/or the recipes of preparation. From activity and selectivity values measured for a set of test reactions (Fig. la) corresponding performance profiles (Fig. lb)) can be derived which can be understood as catalytic fingerprints for individual catalysts. Thus, performance profiles allow a statistical analysis of similarities (Fig. lb). 

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