Filterability of Erythrocytes

addition of calcium ions (increase in erythrocyte rigidity) 

storing the sample for at least 4 h (cellular ageing, depletion of ADP). 

Single erythrocyte rigidometer (Myrenne, 52159 Roetgen, Germany) 

Blood is collected from Beagle dogs, weighing 12-20 kg, or from rabbits, weighing 1.2-2.5 kg, or from Wistar rats, weighing 150-300 g, or from man. The test subjects receive the test compound by oral, subcutaneous or intravenous administration 15, 60, 90 or 180 min before the withdrawal of blood. 

Following addition of the test compound, the blood samples are incubated at 37 °C for 5 or 30 min. 

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