Filling pores with polypeptide

The pores were either partially or completely filled with polypeptide (Figure 3(b) and (c), respectively) depending on the polymerization time. At 115 °C and for Ih deposition time, hollow structures were obtained with a wall thickness around 80nm. A further increase in polymerization time up to 2h converted these nanotubes into solid nanorods (Figure 3(c)). Besides the polymer formation inside the pores, a thick polypeptide film covered the surfaces of the porous alumina templates. The excess film on top of the alumina templates was removed with a Microtome Ultra Cutter. These nanorods were released from the template... 

Hence, with porous particles, surface interaction will predominantly occur when the polypeptide and protein adsorbates reach the internal surface of the particles, thus enabling the mass balance, rate-limiting steps, and the mass transfer coefficients to be quantitatively and independently described. If it is assumed that the pores of the porous HPLC particles are initially filled with buffer liquid before the adsorption process starts, then the overall mass balance for a polypeptide or protein in a finite bath is given by... 

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