Field-flow fractionation electrophoretic mobility

Table 1. Relationship between X and the physical solute properties using different FFF techniques [27,109] with R=gas constant, p=solvent density, ps=solute density, co2r=centrifugal acceleration, V0=volume of the fractionation channel, Vc=cross-flow rate, E=electrical field strength, dT/dx=temperature gradient, M=molecular mass, dH=hydrodynamic diameter, DT=thermal diffusion coefficient, pe=electrophoretic mobility, %M=molar magnetic susceptibility, Hm=intensity of magnetic field, AHm=gradient of the intensity of the magnetic field, Ap = total increment of the chemical potential across the channel...

FIGURE 20.1 Free-flow electrophoresis separation. Laminar fluid flow carries the organelles perpendicularly to the applied electric held. Organelles migrate in the opposite direction of the electric field according to their electrophoretic mobility. Sample is introduced continually at the inlet and collected in many fractions following... 

Fre- flow electrophoresis is the only continuous electrophoretic separation method. The electrical field is applied perpendicular to a continuous stream of buffer film, which flows through a 0.5-1.0 mm wide cuvette. At one end the sample is injected at a defined location, and at the other end, the fractions are collected by an array of tubes. The different electrophoretic mobilities of the sample components lead to differently strong but constant deviations inside the stream... 

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