Fibroblasts, human, recovery from

Figure 4a. Recovery of nondividing human fibroblasts from cis-DDP toxicity. Stationary-phase human fibroblasts were treated with cis-DDP fA O, 52 /aM and 40 /aM) and were either plated immediately or held in the nondividing...

Work by Maher et al. (51) on the effect of UV irradiation on human fibroblasts has shown that cell survival is lower and mutation rates higher upon UV irradiation in DNA excision-repair-incompetent cells than in normal cells. Normal cells were also observed to survive a usually lethal and mutagenic UV dose upon being held in confluence (nondividing) for a period of time prior to assessment of survival and mutation rate. These results suggest that this recovery was a result of the increased time given DNA repair mechanisms to remove thymine dimers prior to replication. These workers have also demonstrated that the actual number of DNA alkylation sites removed from the DNA of benzo(a)pyrene exposed fibroblasts by repair mechanisms is directly related to cell survival (52). 

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