Fibrin titration

Mihalyi (1954b) studied in a similar way the difference in the titration curves of fibrinogen and of polymerized (clotted) fibrin. By subtracting from this difference the difference described above between fibrinogen and... 

Method of GrUtzner. — Fibrin, reduced to threads as fine as posable, is dipped for several seconds in an ammonlacal solution of carmine, is then dried and is rinsed in pure water. The product, colored red, is dissolved in a solution of pepsin hydrochloride, the more rapidly the richer the liqmd is in enzyme. Therefore, a comparative colorimetric determination is made of the quantity of coloring matter dissolved in a test liquid and in a titrated solution of pepsin, at the end of a given time. The ratio of the shades gives the ratio of the enzymic activities. 

In 1952, an army recruit attempted suicide by taking rat poison containing warfarin. On admission to hospital he had numerous subcutaneous haemorrhages and was suffering from nose-bleeds. His prothrombin time was 54 seconds, compared with a normal value of 14 seconds. (The prothrombin time is the time taken for the formation of a fibrin clot in titrated plasma after the addition of calcium ions and thromboplastin to activate... 

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