Feces protein content

The majority of applications of HPLC in clinical laboratories are based on the use of blood, plasma/ serum, or urine samples, although feces and tissue samples are also used. The high protein content of blood samples ( 70gl in plasma or serum) necessitates some form of sample pretreatment prior to injection onto the column to reduce background interference and to avoid column clogging. The specific preparation steps found most appropriate for the applications listed above will be described in the... 

Normally there is very little fat in the feces. However, fat content in stools may increase because of various fat malabsorption syndromes. Such increased fat excretion is steatorrhea. Decreased fat absorption may be the result of failure to emulsify food contents because of a deficiency in bile salts, as in liver disease or bile duct obstruction (stone or tumor). Pancreatic insufficiency may result in an inadequate pancreatic lipase supply. Finally, absorption itself may be faulty because of damage to intestinal mucosal cells through allergy or infection. An example of allergy-based malabsorption is celiac disease, which is usually associated with gluten intolerance. Gluten is a wheat protein. An example of intestinal infection is tropical sprue, which is often curable with tetracycline. Various vitamin deficiencies may accompany fat malabsorption syndromes. 

In the case of indole, alkaline hydrolysis and putrefaction of proteins result in its formation. This formation in the putrefaction of proteins is presumed to be result of decomposition of tryptophan. The formation of indole from albumin may be stopped by the addition of lactose, while other sugars have varying effects on its production. Indole frequently accompanies pus formation and is found in the human liver, pancreas, brain, and bile. Indole, accompanied by its p-methyl homolog, skatole, is found in the feces of humans and animals and in the contents of the intestines. ... 

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