Fast photochemical oxidation of proteins FPOP

Hydroxyl radical oxidative modification, one of the footprinting approaches, utilizes hydroxyl radicals to oxidize certain amino acid side chains followed by extent of modification measurement by MS. For example, fast photochemical oxidation of proteins (FPOP) [35] involves irradiation of protein sample solutions containing hydrogen peroxide by a KrF excimer laser and generation of hydroxyl radicals in the solution. FPOP occurs on the microsecond timescale for the labeling... 

Niu, B Zhang, H Giblin, D Rempel, D.L., Gross, M.L. (2015) Dosimetry Determines the Initial OH Radical Concentration in Fast Photochemical Oxidation of Proteins (FPOP). J. Am. Soc. Mass Spectrom. 26 843-846. 

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