Fast photochemical oxidation of proteins

Figure 14.1 Analytical strategies In epitope mapping. Strategies in epitope mapping can be classified into three categories immunochemical analysis, structural analysis, and computational analysis. Each category can provide complementary Information for other strategies. Abbreviations ELISA, enzyme-linked immunosorbent assay EPOP, fast photochemical oxidation of proteins HX, hydrogen exchange MS, mass spectrometry...

Hydroxyl radical oxidative modification, one of the footprinting approaches, utilizes hydroxyl radicals to oxidize certain amino acid side chains followed by extent of modification measurement by MS. For example, fast photochemical oxidation of proteins (FPOP) [35] involves irradiation of protein sample solutions containing hydrogen peroxide by a KrF excimer laser and generation of hydroxyl radicals in the solution. FPOP occurs on the microsecond timescale for the labeling... 

Chen, J., Rempel, D.L., Gau, B.C., Gross, M.L. (2012) Fast Photochemical Oxidation of Proteins and Mass Spectrometry FoUow SubmiUisecond Protein Folding at the Amino-Acid Level. Journal of the American Chemical Society, 134 (45), 18724—18731. 

Niu, B Zhang, H Giblin, D Rempel, D.L., Gross, M.L. (2015) Dosimetry Determines the Initial OH Radical Concentration in Fast Photochemical Oxidation of Proteins (FPOP). J. Am. Soc. Mass Spectrom. 26 843-846. 

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