False-negative staining

Sensitivity of immunological stain = 96% = 0.96 False-negative error rate of the test = 4% = 0.04 Specificity of the test = 94% = 0.94 False-positive error rate of the test = 6% = 0.06 Prevalence of effect in the tissues = 1% = 0.01... 

Histology Microbiologic examination using various stains Gold standard >95% sensitive and specific permits classification of gastritis results are not immediate not recommended for initial diagnosis tests for active HP infection antibiotics, bismuth, and PPIs may cause false-negative results... 

The test battery serves as a rapid screening approach to identify an optimal protocol for each antibody-antigen to be tested. The goal is to establish the maximal retrieval level for formalin-masked antigens after undefined fixation times in order to standardize immuno-staining results. In addition, the use of a test battery may identify false-negative or false-positive AR-IHC staining results. 

Progesterone nuclear staining by the IHC method is more heterogeneous than ER and may be a cause of false-negative results, especially in core biopsies or needle aspirates. We have seen similar results on some core biopsies as a result, if a negative ER and PR result on a core biopsy specimen is obtained, one should repeat the test on the excisional breast specimen. 

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