Fabaceous crude drugs

Although OGs are widely distributed in the fabaceous family, fabaceous crude drugs used as anti-hepatitis were limited. Therefore, a qualitical analysis seemed to be important. In order to confirm the constitution of OGs, HPLC profile analyses of the total OG fraction for some crude drugs were performed, Fig. (4). The test samples were classified into two groups one is used as anti-hepatitis (Abri Herba and Puerariae Flos) and the other one is not for such use (the roots of Lupinus polyphyllus and Campylotropis hirtella). 

Fig. (4). HPLC Profiles for Total OG Fractions of Some Fabaceous Crude Drugs HPLC conditions were as follows column, Nova-Pak Cl8 (4 mm, 8 x 100 mm) with Radial-Pak RCM 8 x 10 module, solvent A, H20 TFA=100 0.05 (v/v) solvent B, CH,CN H20 TFA=60 40 0.05(v/v). Elution was done with the following process 0—>83% solvent B (5 min), 83% solvent B (5 min), 83—>100% solvent B (5 min), 100% solvent B (5 min). Flow rate was 1 ml/min. Detection was done by UV at 205 nm. Sample of 50 [i.1 of total OG(l mg/ml in MeOH) were injected.

---

See also in sourсe #XX -- [ ]

---