Extraction from glucuronoxylan

A total volume of 43 mL pyridine/HAc/H20 (9 1 4) was added to a sample of glucuronoxylan (Ig). After extraction under continuous stirring for Ih at room temperature, the sample was centrifuged at 4000 rpm and the supernatant decanted. The pellet was treated once more with a similar volume of the extractant. The supernatants were combined and chloroform was added (1 1.3). After separation of the chloroform phase, the water phase was washed once more with a similar volume of chloroform. The chloroform phases were combined and all phases were dried by rotavaporation. To remove pyridine from the phases, all fractions were sequentially washed by ethanol-toluene-ethanol, adapted from Lundquist et al. (1990) 28). 

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