Extraction and Dansylation of Amino Acids

Thin-Layer Chromatographic Separation of Dansyl Amino Acids 

Separated dansyl ammo acids are visualized under UV light Spots are marked and removed. Elution of the dansyl amino acid from the chromatogram is then accomplished using a strong organic base such as triethylamine or hyamine hydroxide. Hy-amine hydroxide is reportedly better than solvents or solvent mixtures such as n-butyl acetate methanoI, which do not effectively elute dansyl glutamate and dansyl aspartate (Joseph and Halhday, 1975) 

---