Extracting Protection Factors by HX Modeling

The methodologies described so far are suitable for extracting information from one peptide at a time. A typical HX-MS dataset, however, often contains hundreds of overlapping peptides. Taking advantage of all information contained in these peptides may increase the spatial resolution of the HDX result but requires major efforts. Therefore, a more comprehensive approach is required to include all the peptides in the data analysis. Several fairly successful attempts have been made in developing such a tool [16, 20, 34, 35]. 

Clearly, the 20 solutions do not match one another, indicating the HX-MS data does not contain enough information to accurately define each protection factor. However, in some regions when many overlapping peptides are present, protection factors can be determined to a single-residue resolution, as indicated in the residue ranges where all 20 solutions match one another. To get a more clear 

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Hydrogen Exchange Mass Spectrometry of Proteins 7.3.5 Extracting Protection Factors by HX Modeling... 

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