Extractants, isotope coded

Wang, J. H. (2002). Add-labile isotope-coded extractants a class of reagents for quantitative mass spectrometric analysis of complex protein mixtures. Anal. 

Zhou, H. Ranish, J.A. Watts, J.D. Aebersold, R. Quantitative proteome analysis by solid-phase isotope tagging and mass spectrometry. Nat. Biotechnol. 2002, 20, 512-515. Qiu, Y. Sousa, E.A. Hewick, R.M. Wang, J.H. Acid-labile isotope-coded extractants a class of reagents for quantitative mass spectrometric analysis of complex protein mixtures. Anal. Chem. 2002, 74, 4969—4979. 

A more recent separation technique uses the so-called isotope coded affinity tags (ICAT Applied Biosystems, Foster City, CA). In this technique, a tag comprising a stable isotopically labeled marker and a biotin molecule is reacted with the protein extract from a perturbed sample whilst an unlabelled tag is used to mark proteins from a control sample. Following purification on an affinity column to isolate the biotin labeled peptides the biotin is cleaved leaving a mixture of tagged peptides. These either have a labeled (clinical sample) or unlabelled marker (control). The mixture is subject to LC-MS(/MS)... 

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