Externally referenced cell

Fig. 4,1. Coaxial assembly in an NMR cell with an external referencing-internal lock configuration...

A typical layout editor is fully hierarchical, with the capability to handle any number of layers, cells with multiple layers, and cells within cells. One word of caution is that when you import a cell from one design into another, to be sure that the cell is copied over rather than externally referenced. A cell that is externally referenced will not be available to another user who is working with your layout. Usually an option is given when importing to either copy or reference the cell. Be sure to copy it rather than referencing it. 

NMR spectra at 80 MHz were recorded on the Bruker WM200 spectrometer at the MRC Biomedical NMR Centre, Mill Hill, using 15 mm tubes and ca. 4 ml of sample. Typically, 3 ml of packed red cells, previously washed twice with saline solution, were resuspended in 1 ml of D20-saline. Compounds which were insoluble in water were added in ca. 5-20 il of MeOH. Spectra are usually the result of ca. 1 hour accumulations 2000 pulses, 1.8 sec pulse repetition time, 16 K computer points, 45 pulse, and peaks are referenced to external H3PO4 (85% in H20)/15% D2O. 

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