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Extension from an end-labelled primer

Maxam-Gilbert load buffer 80% deionised formamide 10 mM NaOH 1 mM EDTA 0.02% xylene cyanol 0.02% bromophenol blue dNTP 2.5 mM A MV reverse transcriptase 5 -[32P]primer ( 3000 Cilmmol) [Pg.133]

Add 4 xl extension mixture (made of 0.4 julI 25 x RT buffer, 0.8 jil 2.5 mM of each dNTPs, 2.8 xl H20 and 1 unit AMV reverse transcriptasea) to the annealed primer/template solution. [Pg.134]

Dissolve the washed and dried pellet in Maxam-Gilbert load buffer.b [Pg.134]


See other pages where Extension from an end-labelled primer is mentioned: [Pg.133]   


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