Experimental Support for the Sorption and Displacement Processes

The container was sealed with a serum cap and thermostatted at 25°C. 100 mg aliquots of the solute were added sequentially to the mixture by means of a hypodermic syringe. After each addition, the container was shaken, thermal equilibrium allowed to become established over a period of about 30 minutes and then a 5 pi sample of the solvent taken for GC analysis. Corrections were made for 

It should be first noted that the curves relating the concentration of ethyl acetate in the solvent mixture and on the stationary phase are straight and horizontal. As the initial concentration of ethyl acetate in mobile phase was 0.35 %w/v, the volume of mobile phase was 100 ml and the mass of silica was 10 g. It follows that, although a total of about 1.2 g of solute was added to the system, about a third of which resided on the silica surface, neither anisole nor nitrobenzene displaced any ethyl acetate from the silica gel. 

Marcel Dekker, Ii 270 Madison Avenue, New York, New York IOC 

Em is the Mass of Ethyl Acetate in Mobile Phase Sni is the Mass of Solute in Mobile Phase Es is the Mass of Ethyl Acetate on Silica Gel Ss is the Mass of Solute on the Silica Gel 

In addition, the concentration of solute in both phases increased almost linearly with the amount of solute added. This would indicate that the system was operating over that part of the adsorption isotherm that was linear. Thus, the solute was interacting 

---