Experimental Approaches and Analytical Techniques in Studies on Bleaching Herbicides

EXPERIMENTAL APPROACHES AND ANALYTICAL TECHNIQUES IN STUDIES ON BLEACHING HERBICIDES 

A variety of techniques has been used to identify the mode of action of bleaching herbicides on carotenoid biosynthesis and to discover the functional groups on such compounds which are prerequisites for inhibitory activity. The trivial names of herbicides are used in the following sections, and their systematic names are listed in the Appendix at the end of the book. 

Carotenoid Analyses of Higher Plants, Algae, and Cyanobacteria 

In order to establish that a compound is inhibiting carotenoid biosynthesis at a particular step, it is necessary to extract, identify, and quantify the carotenoids from control and treated tissue. Traditionally, this has been achieved by extracting the lipids, separating the carotenoids 

Several tissues have, been used in such investigations. Higher plant seedlings, grown in a light/dark cycle or in total darkness, have often been 

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