Experiment 47 HPLC Determination of Caffeine and Sodium Benzoate in Soda Pop

Experiment 47 HPLC Determination of Caffeine and Sodium Benzoate in Soda Pop 

Soda pop samples must be filtered and degassed. Prepare samples by vacuum filtering through paper 0.45-/rm filters, and then fill small labeled vials. 

Prepare 50 mL of a stock standard solution that is 2.0 mg/mL in caffeine and 5.0 mg/mL in sodium benzoate. Use an analytical balance to weigh the chemicals and a clean 50-mL volumetric flask for the solution. Use distilled water as the diluent. Shake well. 

Prepare calibration standards that are 0.05, 0.10, 0.15, and 0.20 mg/mL in caffeine from the stock standard. Use 25-mL volumetric flasks and measuring pipets or pipetters suggested by your instructor. Calculate the concentration of sodium benzoate in each of these solutions. 

Filter each calibration standard, using the syringe filtering equipment, into small labeled vials. 

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