Examples of quantitation by AES

In order to measure a sample by AES a calibration curve is constructed by aspirating solutions of known concentration into the flame. 

Standard solutions of sodium chloride (NaCl) and potassium chloride (KCl) in water were prepared and diluted appropriately to give a calibration curve across the working range across the range of the instrument (ca 0.05-1 mg/100 ml). The assay was then carried out by diluting the infusion until its concentration was close to that at the mid-point of the calibration series. Water is used as a blank. The following results were obtained  

Both standards were transferred to the same 1000 ml volumetric flask and diluted to 1000 ml. 

Amount of Na mg/100 ml Flame photometry reading Amount of K mg/100 ml Flame photometry reading 

The rest of the points in the calibration series are simply x 2, x 3, x 4 and x 5. These values give the concentrations in Table 6.1. 

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