Example A Hypothetical Enzyme

FIGURE 8.7 Typical data for enzyme kinetics, similar to data for pancreatic carbox3fpeptidase (see Ref. [13]) using synthetic values to show Vmax = 0.090 mM/s and iTM = 6.50 mM at [S] = 0.045 mM. 

We have added two calculated points after the best line was fitted to the data, so that the x- and y-intercepts show up as points on the graph using the Michaelis-Menten equation  

we see that enzyme kinetics uses the steady-state approximation and the double-reciprocal plot to provide a robust approach to study the reaction and Km is a useful concept that tells us what concentration of the substrate will give one half of the maximum rate. 

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