Evolution-mimicking Algorithm for the Improvement of Aptamers

An EMA consists of selection via an inhibition assay, crossover, and mutation of the oligonucleotide sequence in silica. Eirst, a set of 10 DNA sequences, each of which contains a portion of the original aptamer sequence as well as a randomized region, is generated by a computer and synthesized chemically. The inhibitory activity of each aptamer for the target enzyme is measured and 

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