Evaporative fight scattering detectors

Several detectors used in high-performance liquid chromatography (HPLC) and in supercritical fluid chromatography (SEC) can be connected to the CCC column to detect solutes and thus follow separation. They can be, for instance, fluorimeters (very sensitive and used without modifications in CCC), UV-Visible spectroscopes, evaporative fight scattering detectors, atomic emission spectroscopes, etc. Some detectors give more information than the detection of the solute, such as stmctural information of separated components, as in infrared spectroscopy, " mass spectrometry,or nuclear magnetic resonance. These detectors are... 

Evaporative fight scattering detection can be used as a imi-versal detector for LC. Its operation includes the nebufization of the eluent in the nebulizer, solvent evaporation in the drift tube, and scattered fight detection at the fight scattering chamber. Experimental conditions which can be adjusted in most ELSD systems to optimize the detector sensitivity are the nebulizer gas flow rate, mobile phase flow rate, and drift tube temperature. The detector response is non-linear, but can be used in quantitative work if a calibration curve is obtained. 

Jaaskelainen, I. Urtti, A. Liquid chromatography determination of liposome components using a fight-scattering evaporative detector, J.Pharm.BiomedAnal., 1994,12, 977-982. 

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