Evaluation of Isoprene Biosynthetic Process from Different Substrates

Evaluation of Isoprene Biosynthetic Process from Different Substrates 

Conversion of glucose, the major fermented sugar, into isoprene is limited by either the net-gained ATP in the MEP pathway or the precursor acetyl-Co A in the 

Growth on glycerol, which is a more reduced substrate than glucose, is a different story. The MEP pathway can produce isoprene in an amount equal to the 

More interestingly, fatty acids, the most reduced substrate, produce the highest theoretical yield in both MEP and MVA pathways (Table 16.1). Because the intermediate product of the fatty acid -oxidation is acetyl-CoA, which is the main precursor for MVA, the MVA pathway achieves the highest yield (0.72 g g ) and so is the best pathway for isoprene production from fatty acids. As is known, the precursors for the MEP route are Pyr and G3P. Thus, Pyr and G3P must be 

Pathway Substrate Substarte (mol) Isoprene Theoretical (mol) yield (mol mol Theoretical ) yield(gg M ATP (mol) NAD(P)H (mol) Acetate (mol) Net-gained ATP (mol) 

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