Eukaryotes Chlamydomonas reinhardtii

Thecells of protozoa (Docampo and Moreno, 2001) and alga Chlamydomonas reinhardtii (Ruiz etal, 200 lb) possess specific PolyP and Ca2+ storage organelles, i.e. acidocalcisomes, which are similar to vacuoles in some properties, especially in the presence of protonpumping pyrophosphatase. These organelles act as phosphate storage systems for the above lower eukaryotes. 

NH4 assimilation occurs by the action of GS and GOGAT (Wheeler, 1983 Falkowski, 1983). The source of NH4 may be externally suppfied or internally as a product of NO3 reduction or photorespiration. Consequently GS activity gives a measure of both regenerated (based on NH4) and total productivity (based on NO3 and NH4) and is commonly measured in studies of algal physiology (e.g., Slawyk et al, 1997 Slawyk and Rodier, 1986 Rees et al, 1995). Two different isoforms of eukaryotic algal GS proteins have been described based upon their size GS II that is found in eukaryotes (Chen and SMow, 1996 in Chlamydomonas reinhardtii Pesole et al, 1991) and GS III which has been identified in diatoms and some other... 

Devriese, M., Tsakaloudi, V., Garbayo, I., Leon, R., Vilchez, C., and Vigara, J. (2001). Effect ofheavy metals on nitrate assimilation in the eukaryotic microalga Chlamydomonas reinhardtii. Plant Physiol. 

Nuclear-gene targeting by using single-stranded DNA avoids illegitimate DNA integration in Chlamydomonas reinhardtii. Eukaryotic Cell, 4 (7),... 

S. (2003) Copper response element and Crrl-dependent Ni " -responsive promoter for induced, reversible gene expression in Chlamydomonas reinhardtii. Eukaryotic Cell, 2 (5), 995-1002. 

Goodson, C., Roth, R., Wang, Z.T., Goodenough, U., 2011. Structural correlates of cytoplasmic and chloroplast hpid body synthesis in Chlamydomonas reinhardtii and stimulation of hpid body production with acetate boost. Eukaryotic Cell 10, 1592—1606. 

Lawson, M.A., Zacks, D.N., Derguini, F., Nakanishi, K., and Spudich, J.L., Retinal analog restoration of photophobic responses in a blind Chlamydomonas reinhardtii mutant Evidence for an archae-bacterial like chromophore in a eukaryotic rhodopsin, Biophys. /., 60, 6,1490, 1991. 

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