Ethylchloro carbonate

In the same way alanyl-alanine and alanyl-alanine ester, which yielded caibethoxyl-alanyl-alanine ester when treated with ethylchloro-carbonate, can be obtained from alanine anhydride, and leucyl-leucine from leucine anhydride or leucinimide, which was first obtained in 1849 regarded as occurring in the protein molecule, by... 

Acetylenic Grignard reagents react with carbon dioxide (CO2) to yield carboxylic acids of the type RC CCOOH. The reactions with ethylcarbonate [CO(OEt)2] or ethylchloro-... 

A simple method for the attachment of most reactants possessing free amino groups, i.e., oligopeptides and haptens (Weigand et al., 1981 Suter, 1982) is the pretreatment of polystyrene with GA (Barrett, 1977). Polystyrene is activated at a relatively low pH where GA has no strong tendency to react or to form polymers (Table 13.1). After the addition of the immunoreactant, the pH is raised to 8 or 9.5 with 100 mM carbonate (Dobbins Place and Schroeder, 1982), to increase the reactivity of GA. Either GA or ethylchloro-... 

EthyImereaptoacetate. Chloroacetylchloride (15 ml) was added to ethanol (100 ml). Potassium carbonate was added and the solution was stirred at room temperature for 1 hour. The potassium carbonate was filtered off. Excess ethanol was stripped on a rotary evaporator. The residue was added to 40 ml of toluene and the solution was washed with water. The toluene was stripped on an evaporator, yielding 15 ml of ethylchloro-acetate. 

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